Automatic determination of lactic acid in blood.
نویسندگان
چکیده
An automatic reaction rate method is described for the determination of lactic acid in blood. The L ( + ) -lactic acid is selectively oxidized in the presence of lactic dehydrogenaseand diphosphopyridinenucleotide to form an absorbing species. The time required for the formation of a fixed amount of DANH is measuredautomatically and related directly to the lactic acid concentration. The method is sensitive, more rapid than purely chemical procedures, and relative errors are only about 1-3% for the range 6-200 p.gof lactic acid. The coefficient of variation for the determination of lactic acid in 0.2 ml of blood is 3%. Measurement times vary from a few seconds to about 2 mm.
منابع مشابه
L(+) lactic acid production and separation from dairy wastes(whey):in situe separation of lactic acid using lon-exchange resins in automatic control of PH.
Whey with a large amount of BOD(50000 PPM) is a dangerous environmental pollutant.this important source of lavtose(4-5%) is A USEFULL SUBSTRATE FOR A RANGE of fermentation processes.lacitic acid with swvwral applications in industries is one of these products.Specially L(+) isomer of this acid worthing 10 times as much as the mixture of L&D,is used in medical purposes such as absorbable surgica...
متن کاملDetermination of antibiotic resistance of lactic acid bacteria isolated from traditional Turkish fermented dairy products
In this study, the antibiotic resistance (AR) of lactic acid bacteria (LAB) isolated from traditional Turkish fermented dairy products was investigated. Yogurt, white cheese, tulum cheese, cokelek, camız cream and kefir as dairy products were collected from various supermarkets. Lactic acid bacteria such as Lactobacillus spp., Streptococcus spp., Bifidobacterium spp., and Enterecoccus spp. were...
متن کاملThe Effects of the Different Frequencies of Whole-Body Vibration after a Strenuous Activity on Blood Lactic Acid
Background. Fatigue has an undesirable effect on the continued exercise performance and decreases athlete's activity. Fast lactic acid disposed is high of importance for athletes. Objectives. Therefore, the present article determines the effects of the different frequencies of whole-body vibration after a strenuous activity on blood lactic acid. Methods. 60 athletes were chosen from sel...
متن کاملDetermination the number of Lactic Acid Bacteria and Yeasts in the combination of traditional yoghurts of villages of East-Azerbaijan- province
Abstract Background and objectives: Yoghurt is a milky, fermented and semi-solid production that is produced by Microorganism starters. These microorganisms are known as lactic acid bacteria, which are responsible for the formation of the tissue, scent and flavor of yoghurt. Their presence in digestive system makes people healthy. The aim of this study was determination of the number of Lactic ...
متن کاملThermal processing determination time for fermented and acidified indigenes Iranian vegetables
Vegetables may be preserved by fermentation, direct acidification, or a combination of these, along with pasteurization, to yield products with an extended shelf life and enhanced safety. Effect of lowering the pH, either by adding acid or lactic fermentation, on thermal process requirements for canned vegetables was investigated. Malic acid was preferred for acidification of canned vegetables...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 15 10 شماره
صفحات -
تاریخ انتشار 1969